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human fetal lung fibroblast cell line imr90 (ATCC)

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ATCC human fetal lung fibroblast cell line imr90
Human Fetal Lung Fibroblast Cell Line Imr90, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 2171 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/imr90+human+fetal+lung+fibroblast+cells/IMR-90/pm40898359-134-7-17
Average 99 stars, based on 2171 article reviews

human fetal lung fibroblast cell line imr90 - by Bioz Stars, 2026-09

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Article Title: Bimetallic titanocene-gold phosphane complexes inhibit invasion, metastasis, and angiogenesis-associated signaling molecules in renal cancer
Article Snippet: VEGF Assay Caki-1 cells treated in vitro with either IC 20 concentrations of bimetallic Ti-Au Titanocref ( 2 ), Titanofin ( 4 ) and monometallic Au cref ( 2 ), and fin ( 3 ) and Auranofin or 0.1% DMSO were lysed after 72 hours of treatment, cell culture supernatant was collected and VEGF expression was sassessed by a VEGF human ELISA kit (100663 Abcam). . 4.2.9. .. IMR90 (human fetal lung fibroblast) cells were purchased from ATCC (Manassas, Virginia, USA) and maintained in Dulbecco's modified Eagle's medium (DMEM) (Mediatech) supplemented with 10% FBS, 1% NEAA and 1% Penicillin Streptomycin. .. After 72 hours of treatment, cell culture supernatant was collected, and interleukin expression was determined by Multi-Target ELISA array kit (PathScan Cytokine Antibody Array Kit, Cell Signalling).

Article Title: Bimetallic titanocene-gold phosphane complexes inhibit invasion, metastasis, and angiogenesis-associated signaling molecules in renal cancer.
Article Snippet: Human renal cell carcinoma line Caki-1 was newly obtained for these studies from the American Type Culture Collection (ATCC) (Manassas, Virginia, USA) and cultured in Roswell Park Memorial Institute (RPMI-1640) (Mediatech Inc., Manassas, VA) media containing 10% Fetal Bovine Serum, certified, heat inactivated, US origin (FBS) (Gibco, Life Technologies, US), 1% Minimum Essential Media (MEM) nonessential amino acids (NEAA, Mediatech) and 1% penicillin–streptomycin (PenStrep, Mediatech). .. IMR90 (human fetal lung fibroblast) cells were purchased from ATCC (Manassas, Virginia, USA) and maintained in Dulbecco's modified Eagle's medium (DMEM) (Mediatech) supplemented with 10% FBS, 1% NEAA and 1% Penicillin Streptomycin. .. HUVEC (human umbilical vein endothelial) cells were obtained from ATCC and cultured in Medium 200PRF (Gibco, Life Technologies, US).

Article Title: Customizing Morphology, Size, and Response Kinetics of Matrix Metalloproteinase-responsive Nanostructures by Systematic Peptide Design
Article Snippet: Human clear cell renal cell carcinoma line Caki-1 was newly obtained for these studies from the American Type Culture Collection (ATCC) (Manassas, Virginia, USA) and cultured in Roswell Park Memorial Institute (RPMI-1640) (Mediatech Inc., Manassas, VA) media containing 10% Fetal Bovine Serum, certified, heat inactivated, US origin (FBS) (Gibco, Life Technologies, US), 1% Minimum Essential Media (MEM) nonessential amino acids (NEAA, Mediatech) and 1% penicillin–streptomycin (PenStrep, Mediatech). .. IMR90 (human fetal lung fibroblast) cells were purchased from ATCC (Manassas, Virginia, USA) and maintained in Dulbecco’s modified Eagle’s medium (DMEM) (Mediatech) supplemented with 10% FBS, 1% NEAA and 1% Penicillin Streptomycin. ..

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Journal: iScience

Article Title: Human SKI component SKIV2L regulates telomeric DNA-RNA hybrids and prevents telomere fragility

doi: 10.1016/j.isci.2024.111096

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Article Snippet: Stable clone of adult female human cervical adenocarcinoma HeLa cell line with long telomeres (HeLa1.3 cells); adult male human fibrosarcoma HT1080 cell line overexpressing telomase (HT1080 super-telomerase cells, HT1080-ST) (kindly provided by T. de Lange and J. Lingner, respectively); adult female human osteosarcoma U2OS cell line (ATCC); human embryonic kidney HEK293FT cell line (ATCC); and human female fetal lung fibroblast IMR90 cell line (Coriel Institute) were cultured in DMEM medium supplemented with 10% (v/v) fetal bovine serum (FBS, Sigma-Aldrich, F2442) and maintained at 37°C in 5% (v/v) CO 2 .

Techniques: Virus, Recombinant, Protease Inhibitor, Reverse Transcription, Blocking Assay, Mass Spectrometry, SYBR Green Assay, Flow Cytometry, Imaging, Mutagenesis, Cell Cycle Assay, shRNA, Software

Fig. 2 LPS-mediated caspase-4 induced senescence is independent of inﬂammasome priming. A CASP4, CASP1 or RASG12V were overexpressed in IMR90 cells, IL1A and IL1B mRNA relative expression levels were quantiﬁed by RT-qPCR. Graph bars, error bars and dots represent respectively the mean ± s.e.m. and the individual values of 3 independent experiments. Statistical analysis was performed using one-way analysis of variance (ANOVA). B Cells were treated as shown in Fig. 1A. CASP1 or CASP4 expression was targeted by shRNA prior to LPS transfection with 0.1 μg LPS. IL1B mRNA relative expression was quantiﬁed by RT-qPCR 48 h after LPS transfection. Graph bars, error bars and dots represent respectively the mean ± s.e.m. and the individual values of 3 independent experiments. Statistical analysis was performed using one-way analysis of variance (ANOVA). C–E IMR90 cells were infected with CASP4 or RASG12V expression vectors or empty vector (vector) control. After 3 h treatment with Pam2CSK4, cells were transfected with 0.1 μg LPS. IL1B mRNA relative expression (C) and BrdU incorporation (D) were measured by IF and RT-qPCR respectively 48 h after LPS transfection. E SA-β-Galactosidase activity was determined 4 days after LPS transfection. Representative images for SA-β-Galactosidase activity are shown. Graph bars, error bars and dots represent respectively the mean ± s.e.m. and the individual values of 3 independent experiments. Statistical analysis was performed using one-way analysis of variance (ANOVA). ****P < 0.0001, ***P < 0.001, **P < 0.01, and *P < 0.05. Scale bar = 0.1 mm as indicated.

Journal: Cell death and differentiation

Article Title: Cytoplasmic innate immune sensing by the caspase-4 non-canonical inflammasome promotes cellular senescence.

doi: 10.1038/s41418-021-00917-6

Figure Lengend Snippet: Fig. 2 LPS-mediated caspase-4 induced senescence is independent of inﬂammasome priming. A CASP4, CASP1 or RASG12V were overexpressed in IMR90 cells, IL1A and IL1B mRNA relative expression levels were quantiﬁed by RT-qPCR. Graph bars, error bars and dots represent respectively the mean ± s.e.m. and the individual values of 3 independent experiments. Statistical analysis was performed using one-way analysis of variance (ANOVA). B Cells were treated as shown in Fig. 1A. CASP1 or CASP4 expression was targeted by shRNA prior to LPS transfection with 0.1 μg LPS. IL1B mRNA relative expression was quantiﬁed by RT-qPCR 48 h after LPS transfection. Graph bars, error bars and dots represent respectively the mean ± s.e.m. and the individual values of 3 independent experiments. Statistical analysis was performed using one-way analysis of variance (ANOVA). C–E IMR90 cells were infected with CASP4 or RASG12V expression vectors or empty vector (vector) control. After 3 h treatment with Pam2CSK4, cells were transfected with 0.1 μg LPS. IL1B mRNA relative expression (C) and BrdU incorporation (D) were measured by IF and RT-qPCR respectively 48 h after LPS transfection. E SA-β-Galactosidase activity was determined 4 days after LPS transfection. Representative images for SA-β-Galactosidase activity are shown. Graph bars, error bars and dots represent respectively the mean ± s.e.m. and the individual values of 3 independent experiments. Statistical analysis was performed using one-way analysis of variance (ANOVA). ****P < 0.0001, ***P < 0.001, **P < 0.01, and *P < 0.05. Scale bar = 0.1 mm as indicated.

Article Snippet: HEK293T, MEFs, CAPAN-1, PSN-1, A549, HCT116, THP-1, and IMR90 female human fetal lung fibroblast cells were obtained from American Type Culture Collection.

Techniques: Expressing, Quantitative RT-PCR, shRNA, Transfection, Infection, Plasmid Preparation, Control, BrdU Incorporation Assay, Activity Assay

Fig. 3 Caspase-4 mediated regulation of senescence is independent of its catalytical function. A–C IMR90 cells were infected with wild- type (WT) CASP4, catalytically inactive (C258A) CASP4 or the empty vector (vector). Overexpression of RASG12V was used as a positive control for the induction of senescence. A Caspase-4 and IL-1β expression levels were investigated by immunoblotting. β-Actin immunoblot was performed for loading control. B Caspase-4 protein expression levels and BrdU incorporation measured by immunoﬂuorescence, as well as SA-β-Galactosidase activity were assessed 4 days after equal number of cells were seeded. C Relative cell content (left) was quantiﬁed 15 days after equal number of cells were seeded; representative images (right) of crystal violet stained cells are shown. Graph bars, error bars and dots represent respectively the mean ± s.e.m. and the individual values of 3 independent experiments. Statistical analysis was performed using one-way analysis of variance (ANOVA). D–G (D) Schematic representation of the experiment shown in D-F and Supplementary Fig. 3G–H. IMR90 cells were infected with wild-type (WT) CASP4, catalytically inactive (C258A) CASP4 or the empty vector (vector) prior to transfection with 1 μg LPS. (E) Cell viability was measured 24 h after LPS transfection, (F) BrdU incorporation was measured by immunoﬂuorescence 48 h after LPS transfection and (G) SA-β-Galactosidase activity was determined 4 days after LPS transfection. Graph bars, error bars and dots represent respectively the mean ± s.e.m. and the individual values of 3 independent experiments. Statistical analysis was performed using two-tailed Student’s t test. ****P < 0.0001, ***P < 0.001, **P < 0.01, and *P < 0.05. ns, not signiﬁcant.

Journal: Cell death and differentiation

Article Title: Cytoplasmic innate immune sensing by the caspase-4 non-canonical inflammasome promotes cellular senescence.

doi: 10.1038/s41418-021-00917-6

Figure Lengend Snippet: Fig. 3 Caspase-4 mediated regulation of senescence is independent of its catalytical function. A–C IMR90 cells were infected with wild- type (WT) CASP4, catalytically inactive (C258A) CASP4 or the empty vector (vector). Overexpression of RASG12V was used as a positive control for the induction of senescence. A Caspase-4 and IL-1β expression levels were investigated by immunoblotting. β-Actin immunoblot was performed for loading control. B Caspase-4 protein expression levels and BrdU incorporation measured by immunoﬂuorescence, as well as SA-β-Galactosidase activity were assessed 4 days after equal number of cells were seeded. C Relative cell content (left) was quantiﬁed 15 days after equal number of cells were seeded; representative images (right) of crystal violet stained cells are shown. Graph bars, error bars and dots represent respectively the mean ± s.e.m. and the individual values of 3 independent experiments. Statistical analysis was performed using one-way analysis of variance (ANOVA). D–G (D) Schematic representation of the experiment shown in D-F and Supplementary Fig. 3G–H. IMR90 cells were infected with wild-type (WT) CASP4, catalytically inactive (C258A) CASP4 or the empty vector (vector) prior to transfection with 1 μg LPS. (E) Cell viability was measured 24 h after LPS transfection, (F) BrdU incorporation was measured by immunoﬂuorescence 48 h after LPS transfection and (G) SA-β-Galactosidase activity was determined 4 days after LPS transfection. Graph bars, error bars and dots represent respectively the mean ± s.e.m. and the individual values of 3 independent experiments. Statistical analysis was performed using two-tailed Student’s t test. ****P < 0.0001, ***P < 0.001, **P < 0.01, and *P < 0.05. ns, not signiﬁcant.

Article Snippet: HEK293T, MEFs, CAPAN-1, PSN-1, A549, HCT116, THP-1, and IMR90 female human fetal lung fibroblast cells were obtained from American Type Culture Collection.

Techniques: Infection, Plasmid Preparation, Over Expression, Positive Control, Expressing, Western Blot, Control, BrdU Incorporation Assay, Activity Assay, Staining, Transfection, Two Tailed Test

Fig. 4 The caspase-4 noncanonical inﬂammasome is activated in oncogene-induced senescence. A IMR90 cells were infected with RASG12V

Journal: Cell death and differentiation

Article Title: Cytoplasmic innate immune sensing by the caspase-4 non-canonical inflammasome promotes cellular senescence.

doi: 10.1038/s41418-021-00917-6

Figure Lengend Snippet: Fig. 4 The caspase-4 noncanonical inﬂammasome is activated in oncogene-induced senescence. A IMR90 cells were infected with RASG12V

Article Snippet: HEK293T, MEFs, CAPAN-1, PSN-1, A549, HCT116, THP-1, and IMR90 female human fetal lung fibroblast cells were obtained from American Type Culture Collection.

Techniques: Infection

Fig. 6 Caspase-4 contributes to the arrest in cell proliferation in OIS. A IMR90 ER:STOP and ER:RAS cells were transfected with control (NTP), two individual CASP4-targeting siRNAs (CASP4-1 and CASP4-2) or a pool of 4 different siRNA sequences targeting CASP4 (CASP4-p), and treated with 4OHT or not as indicated. BrdU incorporation was measured by immunoﬂuorescence 5 days after 4OHT addition. Graph bars, error bars and dots represent respectively the mean ± s.e.m. and the individual values of 3 independent experiments. Statistical analysis was performed using two-tailed Student’s t test. B, C IMR90 cells were transduced with an empty retroviral vector (vector) or shRNAs targeting either CASP4 (shCASP4) or TP53 (shP53). B Lysates were subjected to immunoblotting analyses with the indicated antibodies eight days after 4OHT addition. C On day 0, equal number of cells were subjected to 4OHT treatment. Fifteen days after 4OHT addition, plates were ﬁxed and stained with crystal violet. Crystal violet was extracted and used to quantify cell content. Graph bars, error bars and dots represent respectively the mean ± s.e.m. and the individual values of 4 independent experiments. Statistical analysis was performed using two-tailed Student’s t test. D Related to Fig. 5A. DEG analysis was performed between control ER:STOP and CASP4-knockdown ER:RAS compared to control ER:RAS after 5 days of 4OHT treatment. Heatmap of the log2FC values from the indicated genes. E IMR90 ER:STOP and ER:RAS cells were transfected with control (NTP), CASP1 or CASP4- targeting siRNAs and treated with 4OHT during 4 days. Cell lysates were subjected to immunoblotting analyses with the indicated antibodies. F IMR90 ER:RAS cells were transfected with control (NTP), CASP1 or CASP4-targeting siRNA and mRNA relative expression of the indicated genes was quantiﬁed by RT-qPCR after 5 days of 4OHT treatment. Graph bars, error bars and dots represent respectively the mean ± s.e.m. and the individual values of 3 independent experiments. Statistical analysis was performed using one-way analysis of variance (ANOVA). ***P < 0.001, **P < 0.01, *P < 0.05 and ns, not signiﬁcant.

Journal: Cell death and differentiation

Article Title: Cytoplasmic innate immune sensing by the caspase-4 non-canonical inflammasome promotes cellular senescence.

doi: 10.1038/s41418-021-00917-6

Figure Lengend Snippet: Fig. 6 Caspase-4 contributes to the arrest in cell proliferation in OIS. A IMR90 ER:STOP and ER:RAS cells were transfected with control (NTP), two individual CASP4-targeting siRNAs (CASP4-1 and CASP4-2) or a pool of 4 different siRNA sequences targeting CASP4 (CASP4-p), and treated with 4OHT or not as indicated. BrdU incorporation was measured by immunoﬂuorescence 5 days after 4OHT addition. Graph bars, error bars and dots represent respectively the mean ± s.e.m. and the individual values of 3 independent experiments. Statistical analysis was performed using two-tailed Student’s t test. B, C IMR90 cells were transduced with an empty retroviral vector (vector) or shRNAs targeting either CASP4 (shCASP4) or TP53 (shP53). B Lysates were subjected to immunoblotting analyses with the indicated antibodies eight days after 4OHT addition. C On day 0, equal number of cells were subjected to 4OHT treatment. Fifteen days after 4OHT addition, plates were ﬁxed and stained with crystal violet. Crystal violet was extracted and used to quantify cell content. Graph bars, error bars and dots represent respectively the mean ± s.e.m. and the individual values of 4 independent experiments. Statistical analysis was performed using two-tailed Student’s t test. D Related to Fig. 5A. DEG analysis was performed between control ER:STOP and CASP4-knockdown ER:RAS compared to control ER:RAS after 5 days of 4OHT treatment. Heatmap of the log2FC values from the indicated genes. E IMR90 ER:STOP and ER:RAS cells were transfected with control (NTP), CASP1 or CASP4- targeting siRNAs and treated with 4OHT during 4 days. Cell lysates were subjected to immunoblotting analyses with the indicated antibodies. F IMR90 ER:RAS cells were transfected with control (NTP), CASP1 or CASP4-targeting siRNA and mRNA relative expression of the indicated genes was quantiﬁed by RT-qPCR after 5 days of 4OHT treatment. Graph bars, error bars and dots represent respectively the mean ± s.e.m. and the individual values of 3 independent experiments. Statistical analysis was performed using one-way analysis of variance (ANOVA). ***P < 0.001, **P < 0.01, *P < 0.05 and ns, not signiﬁcant.

Article Snippet: HEK293T, MEFs, CAPAN-1, PSN-1, A549, HCT116, THP-1, and IMR90 female human fetal lung fibroblast cells were obtained from American Type Culture Collection.

Techniques: Transfection, Control, BrdU Incorporation Assay, Two Tailed Test, Transduction, Retroviral, Plasmid Preparation, Western Blot, Staining, Knockdown, Expressing, Quantitative RT-PCR

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